Table 1.
Judgment time of proof-of-concept investigation by comparison between MCD and each resistant bacteria.
| organism | Incubation time (h) |
average time (h) | ||||
|---|---|---|---|---|---|---|
| 2 | 3 | 4 | ||||
|
Staphylococcus aureus |
mecA gene |
positive (n = 30) | 0 | 29 | 1 | 3.03 |
| negative (n = 30) |
0 |
28 |
2 |
3.07 |
||
| total (n = 60) | 0 | 57 | 3 | 3.05 | ||
|
Enterobacterales |
ESBL gene |
positive (n = 30) | 6 | 24 | 0 | 2.80 |
| negative (n = 30) |
2 |
27 |
1 |
2.97 |
||
| total (n = 60) | 8 |
51 |
1 |
2.88 |
||
| pAmpC gene |
positive (n = 30) | 6 | 24 | 0 | 2.80 | |
| negative (n = 30) |
2 |
27 |
1 |
2.97 |
||
| total (n = 60) | 8 |
51 |
1 |
2.88 |
||
| Carbapenemase gene |
positive (n = 30) |
6 |
24 |
0 |
2.80 |
|
| negative (n = 30) |
2 |
27 |
1 |
2.97 |
||
| total (n = 60) | 8 | 51 | 1 | 2.88 | ||
MCD: Microcolony Detection method; ESBL: extended spectrum β-lactamase; pAmpC: plasmid mediated AmpC β-lactamase; The MCD used CHROMagar MRSA medium for comparison with mecA gene of staphylococci, chromID ESBL medium for comparison with ESBL and pAmpC gene of Enterobacterales, and CHROMagar mSuperCARBA medium for comparison with carbapenemase producing Enterobacterales.