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. 2020 Oct 17;48(20):11773–11784. doi: 10.1093/nar/gkaa842

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Characterization of the two-hairpin intermediate joint by fluorescence. (A) Workflow of the two-hairpin intermediate joint in the fluorescence reporter system. (B) Fluorescence measurement in the presence of different components after a 20-min incubation. The concentrations for these components: 60 nM trigger strand (T), 60 nM H₁, 60 nM H₂, and 30 nM reporter duplex RepF:RepQ. (C) Kinetic analysis of the computing process under different concentrations of trigger strand (0, 30, 45, 60, 75 and 90 nM, respectively). (D) Comparison of fluorescence signals between the correct (T) and incorrect (T_w) trigger strand after a 24-h incubation. Error bars represent standard deviations derived from at least three independent experiments. Sequences used in this fluorescence assay were listed in Supplementary Table S2.