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Binding of poly-His-tagged UdgX to uracils in single-stranded DNA. Homogeneous polyHis-tagged UdgX protein was reacted with a 6FAM-labeled oligomer containing a single uracil in E. coli uracil-DNA glycosylase reaction buffer (New England Biolabs, A) or a buffer optimized for UdgX (B). E. coli Ung was used in one reaction to confirm that the oligomer contained a uracil.
