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FLAG-UdgX is inhibited by imidazole. (A) Homogeneous FLAG-UdgX protein was reacted with a 6FAM-labeled oligomer containing a single uracil. The reactions were performed in UdgX optimized buffer and the products were separated on a denaturing gel. (B) FLAG-UdgX protein was pre-incubated with imidazole at the concentration shown and then reacted with the 6FAM-labeled oligomer in UdgX buffer. The imidazole was removed by buffer exchange and the products were analyzed on a denaturing gel.
