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UdgX preferentially binds uracils in ssDNA. (A) The 6FAM-labeled oligomer with a uracil (ssU) was annealed to unlabeled complement strands with either a guanine (dsU:G) or adenine (dsU:A) base across from the uracil. FLAG-UdgX was reacted with these substrates in UdgX buffer. (B) Two pmol of the ssU substrate was mixed with different amounts of E. coli strain GM31 or CJ236 DNA and reacted with FLAG-UdgX.
