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. 2020 Jun 19;48(20):11521–11535. doi: 10.1093/nar/gkaa527

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Closure motif of ASY1 functions as a nuclear localization signal (NLS) and is not required for its chromosomal localization. (A) Schematic of different ASY1 versions and ASY3 used in this study. (B) Localization of ASY1:GFP, ASY1^Δclosure:GFP, and ASY1^Δclosure-NLS:GFP in the male meiocytes of asy1 mutants using confocal microscopy. Bar: 10 μm. (C) Localization of closure:GFP in the anther of wildtype using confocal microscopy. The CLOSE-UP shows the magnification of the connective tissue highlighted by the red rectangle. Bar: 10 μm. Arrow indicates the position of male meiocytes. (D) Localization of ASY1:GFP and ASY1^Δclosure-NLS:GFP at different prophase stages in male meiocytes of asy1 mutants. Bar: 5 μm.