Figure 3.
The closure motif is required for the PCH2-mediated removal of ASY1 from synapsed chromosomes. (A and B) Co-immunolocalization of ASY1-NLS:GFP (A) or ASY1Δclosure-NLS:GFP (B) with ZYP1 in male meiocytes of wild-type plants using antibody against GFP and ZYP1. Note that for increased visibility, the brightness of the magnified panel in (B) was enhanced by 40%. (C) Co-localization of ASY1:RFP with ZYP1b:GFP in male meiocytes of wildtype (WT) and ASY1Δclosure-NLS/WT plants using confocal microscopy. All blue and white arrows indicate the non-synapsed and synapsed regions, respectively. Bar: 5 μm.