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. 2020 Jun 19;48(20):11521–11535. doi: 10.1093/nar/gkaa527

Figure 5.

Figure 5.

The PCH2-mediated dissociation of the closure motif of ASY1 from the HORMA domain is required for its efficient nuclear targeting. (A) Localization of ASY1Δ10:GFP and ASY1Δ20:GFP at different meiotic stages in male meiocytes of wildtype (WT) using confocal microscopy. Bar: 10 μm. Red rectangles highlight the areas of the close-ups. (B) Yeast two-hybrid assays for the interaction of the closure motif (ASY1571-596) with ASY1 HORMA domain (ASY11-300) and the domains having either the first 9 (ASY11-300(Δ10)) or 19 (ASY11-300(Δ20)) amino acid deletion at the N-terminus. (C) Yeast two-hybrid interaction assays of the closure motif with ASY1 HORMA domain and the domain harboring two non-phosphorylatable mutations ASY11-300(T142V;T184G)). (D) Localization of ASY1T142V;T184G:GFP at late G2 and early prophase stages in male meiocytes of pch2 mutants using confocal microscopy. Bar: 10 μm. Red rectangles highlight the areas of the close-ups.