Table 1.
Summary of the subcellular localization of different versions of ASY1 used in this study in different plant backgrounds. N/A denotes not analyzed
| Constructs | Background | Source | Subcellular localization | Functionality |
|---|---|---|---|---|
| ASY1:GFP | WT and asy1 | From (18) | Nucleus | Functional |
| ASY1:GFP | pch2 | From (18) | Cytoplasm and nucleus | Functional |
| ASY1:GFP | PCH2E283Q (pch2) | This study | Cytoplasm and nucleus | Functional |
| ASY1Δclosure:GFP | asy1 | This study | cytoplasm | Non-functional |
| ASY1Δclosure-NLS:GFP | pch2 | This study | nucleus | Non-functional |
| ASY1-NLS:GFP | WT and asy1 | This study | Nucleus | Functional |
| ASY1Δ10:GFP | WT | This study | Cytoplasm and nucleus | N/A |
| ASY1Δ20:GFP | WT | This study | Nucleus | N/A |
| ASY1T142V;T184G:GFP | WT | This study | Nucleus | Non-functional |
| ASY1Δclosure:GFP:closure | WT and asy1 | This study | nucleus | Functional |
| ASY1Δclosure:GFP:closure | pch2 | This study | Cytoplasm and nucleus | Functional |
| ASY1Δclosure-ASY31-50-NLS:GFP | pch2 | This study | Cytoplasm and nucleus | N/A |