Figure 5.

Sfp1 and Ifh1 are directly recruited at Cat III promoters. (A) Heat maps showing MNase digestion patterns (left panel) and G/C content (right panel) at RPGs promoters. Signals for a window of −500 to +250 bp relative to the +1 nucleosome (0) are displayed (X-axis). (B) Average plots of MNase digestion patterns (upper panel) and G/C content (lower panel) at three categories of RPGs. Signals for a window of −500 to +250 bp relative to the TSS (bp) (0) are displayed (X-axis). (C) Heat maps showing Sfp1-binding motif and Sfp1 ChEC-seq signal for 150 s of Ca⁺² treatment, or Ifh1 ChEC-seq signal after 150 sec of Ca⁺² treatment at the indicated RPGs promoters. Control for ChEC-seq signal (free-MNase, 20 min following Ca⁺² addition) is used as background control. Average plots of Ifh1, Sfp1, Free-MNase ChEC-seq signal at three categories of RPGs is also shown (lower panel). Signals for a window of −500 to +250 bp relative to the TSS (bp) (0) are displayed (X-axis). (D) Genome browser tracks comparing Sfp1-MNase, Ifh1-MNase and free MNase ChEC-seq signals (blue background) to Sfp1-TAP, Ifh1-Myc and untagged ChIP-seq read counts (yellow background) at RPS28A (Cat III) and RPS30B (Cat II) RPGs. The position of indicated RPGs promoters are shown above of the tracks. (E) Box plots of log₂ ChEC-seq or ChIP-seq signal related to ChEC or ChIP control (free MNase or untagged strains) at promoters of different groups of genes (Cat I, II, III, ribosome biogenesis [RiBi] genes, others [200 randomly chosen protein-coding genes]) for Sfp1-MNase and Ifh1-MNase (ChEC-seq, blue background) or Sfp1-TAP and Ifh1-Myc (ChIP-seq, yellow background), respectively.