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. 2020 Oct 14;48(20):11394–11407. doi: 10.1093/nar/gkaa837

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Characterizing the ability of ZFP57 to protect non-imprinted oocyte-derived methylation from epigenetic reprogramming. (A) Schematic representation of the dCas9-Zfp57[KRAB]-T2A-GFP construct and the microinjection strategy for mouse zygotes (B) The results of nested-multiplex bisulphite PCR coupled with locus-specific pyrosequencing for the maternally methylated imprinted control Airn and the targeted loci Serpinb6a, Sgk1 and Tsc22d1. (C) Confirmation of strand-specific methylation by bisulphite PCR and sub-cloning for the Serpinb6a interval. Each circle represents a single CpG dinucleotide on a DNA strand, a methylated cytosine (•) or an unmethylated cytosine (○).