Figure 5.

Alternative promoter usage and the ZFP57 hap-ASM. (A) Schematic overview of the ZFP57 gene, the location of two transcripts observed in blastocyst -derived RNA-seq datasets and the position of the hap-ASM. Grey exonic regions indicate the KRAB domain sequences. (B) Characterization of the allelic expression in Val11B hES cell line and methylation overlapping the alternative ZFP57 promoter heterozygous for SNP rs3129054 by bisulphite PCR followed by sub-cloning. Each circle represents a single CpG dinucleotide on a DNA strand, a methylated cytosine (•) or an unmethylated cytosine (○). (C) Characterisation of monoallelic expression in the Val11B hES cell line.