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. 2020 Sep 28;64:10.29219/fnr.v64.4240. doi: 10.29219/fnr.v64.4240

Fig. 3.

Fig. 3

Expression of EMT marker genes and migration and invasion of KLF4-knockdown MDA-MB-231 cells after treatment with cyanidin-3-O-glucoside. (A) KLF4 protein expression level in 293T cells transfected either with scrambled or with KLF4 shRNAs. (B) Expression of EMT marker genes in scrambled MDA-MB-231 and in KLF4-knockdown MDA-MB-231 cells in the presence or absence of Cy3G. (C) Representative images (magnification, ×100) and statistical results of Transwell migration assays of KLF4-knockdown and scrambled MDA-MB-231 cells in the presence or absence of Cy3G, P > 0.05. (D) Representative images (magnification, ×100) and statistical results of Matrigel invasion assays of KLF4-knockdown and scrambled MDA-MB-231 cells in the presence or absence of Cy3G, P > 0.05. Both migration and invasion assay results were from three separate cell cultures, and the assays were repeated three or four times. The values are expressed as mean ± SEM.