Figure 4.

Sensitivity of ConvAmp cells toward mCMV infection. (A) The sensitivity of ConvAmp cells in response to mCMV infection was determined. ConvAmp cells were infected with an tRFP-tagged mCMV using indicated MOIs. Frequencies of infected cells were determined by flow cytometry (left, 20 h after infection). Luciferase responses were evaluated 24 h after infection (right). Shown are the mean of duplicates. (B) ConvAmp-eGFP cells were seeded one day prior mCMV infection. Cells were infected with mCMV (MOI 1) for 1 h and subjected to time-lapse microscopy. Time points indicate image acquisition post-infection. Representative pictures of one out of two experiments are shown. Luciferase expression of mCMV infected ConvAmp cells is provided in Supplementary Figure S10.