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NANPs are internalized through endocytic pathway and traffic to the cytosol. Microglia were transfected with 5 nM Cy3-labeled NANPs using L2K (A) or DOTAP (B) for 2 or 4 h. Localization of NANPs (green) within cells was determined by immunofluorescence microscopy for the early endosomal marker, EEA1 (red), and nuclear stain DAPI (blue). Arrow heads indicate NANPs colocalized with EEA1, and arrows indicate NANPs that do not colocalize with EEA1.
