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. 2020 Oct 22;48(20):11785–11798. doi: 10.1093/nar/gkaa908

Figure 5.

Figure 5.

2′F modified NANPs stimulate NF-κB and IRF mediated cytokine production. (A) THP-1 Dual reporter cells were transfected with 5 nM NANPs for 24 h using L2K. Induction of the NF-κB pathways was conducted using Quanti-Blue to monitor SEAP activity. Induction of the IRF pathway was conducted using Quanti-Luc to monitor the activity of secreted luciferase. (B, C) Microglia were transfected with 5 nM NANPs using either L2K (B) or DOTAP (C) for 4 h and cell supernatants were collected 24 h post transfection. Cell supernatants were then analyzed for cytokine production using specific capture ELISAs for IL-6 and IFN-β. Data are represented as the mean ± (SEM) for a minimum of three independent experimental replicates. Asterisks indicate statistical significance compared to L2K, pound symbols indicate statistical significance compared to DcR, dagger symbols indicate statistical significance compared to Dc2′F, double dagger symbols indicate statistical significance compared to Rc2′F (Student's t-test, P-value < 0.05).