Figure 6.

RcR and RcD NANPs stimulate TLR7 responses. (A) Schematic of HEK-Blue TLR reporter cells. Created with Biorender.com (B) HEK-Blue TLR reporter cells were transfected with 5 nM NANPs for 24 h. TLR induced NF-κB activity was evaluated using Quanti-Blue to monitor SEAP activity. Poly (I:C), R848 and ODN 2006 were used as positive controls for TLR3, TLR7 and TLR9, respectively. Data are represented as the mean ± (SEM) for three independent experimental replicates. Asterisks indicate statistical significance compared to L2K (Student's t-test, P-value < 0.05).