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. 2020 Nov 2;48(20):11626–11644. doi: 10.1093/nar/gkaa814

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Subcellular distribution of SINEUP RNAs after overexpression of SINEUP RBPs. (A1, B1) Representative RNA FISH with immunofluorescence images of the subcellular distribution of SINEUP RNAs and SINEUP RBPs in cells overexpressing PTBP1 (+PTBP1) (A1) or HNRNPK (+HNRNPK) (B1). Images for cells co-transfected with EGFP and SINEUP-GFP vectors (left images, A1 and B1) were compared with cells transfected with SINEUP-GFP vector alone (right images, A1 and B1). Bars indicate 5 μm. (A2, A3, B2, B3) Quantitative comparison of SINEUP-GFP RNA distribution between cells overexpressing PTBP1 (A2, A3) or HNRNPK (B2, B3) and cells without overexpression (Cont.). Results for cells co-transfected with EGFP and SINEUP-GFP vectors (A2 and B2) and those transfected with SINEUP-GFP vector alone (A3, B3) are shown. SINEUP RNA signals were detected using Icy Spot Detector. The ratio of spots in the nucleus and the cytoplasm were compared between overexpression and non-overexpression of SINEUP RBPs. *P < 0.05 by Student's t-test. Data are means ± SD of at least 10 independent cell images.