Figure 5.

Solution structure of PaP3 and NV1 TerSs. Ab initio SEC-SAXS electron density of PaP3 (A) and NV1 (B) TerS at FSC resolution of 33.3 Å and 38.1 Å, respectively (Supplementary Table S1). Ribbon diagrams of the crystallographic models are overlaid to the electron densities with Pap3 TerS in orange and NV1 TerS in purple. The black circle in panel B and its relative zoomed-in insertion indicates one HTH in the close conformation. (C) The modeled structure of NV1 TerS with open HTHs is overlaid to the SAXS density. The zoomed-in insertion shows a superimposition of HTHs in the closed (purple) versus open (blue) conformations. The agreement between solution and crystal structures was calculated using the FoxS server (see Supplementary Figure S8).