Table 1.
Biophysical parameters measured using AUC-SV
| PaP3 TerS | NV1 TerS | LUZ24 TerS | ||||
|---|---|---|---|---|---|---|
| AUC-SV | Peak 1 | Peak 2 | Peak 1 | Peak 2 | Peak 1 | Peak 2 |
| Protein concentration (mg ml−1) | 1.2 | 1.3 | n.a. | 2.2 | 2.3 | 2 |
| Apparent sedimentation coef., s (S) | 3.49 ± 0.20 | 3.78 ± 0.02 | n.a. | 3.68 ± 0.03 | 3.14 ± 0.20 | 3.68 ± 0.03 |
| Absolute sedimentation coef., s20,w (S) | 3.97 | 4.28 | n.a. | 4.21 | 3.58 | 4.20 |
| Theoretical monomer M.W. (kDa) | 17.01 | 17.73 | 17.01 | |||
| Estimated M.W. (kDa) | 154.4 ± 2.57 | 152.20 ± 0.57 | n.a. | 164.04 ± 3.12 | 103.7 ± 3.50 | 156.84 ± 2.50 |
| Possible oligomeric state (theoretical M.W., kDa) | Heterogeneous | 9-mer (153.1) | n.a. | 9-mer (159.6) | Heterogeneous | 9-mer (153.0) |
| Frictional ratio, f/f0 | 2.55 | 2.35 | n.a. | 2.47 | 2.15 | 2.39 |
| Abundance in each population (%) | 95 | 61.2 | n.a. | 92.5 | 90.7 | 91.4 |