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. 2020 Oct 14;48(20):e120. doi: 10.1093/nar/gkaa849

Figure 1.

Figure 1.

Binding-dependent digestion of ATP-binding aptamer ATP-33 by T5 Exo. (A) Scheme of T5 Exo digestion of ATP-33 in the absence (left) and presence (right) of ATP. (B) Time course digestion of ATP-33 by T5 Exo in the absence (−) or presence (+) of 250 μM ATP analyzed by polyacrylamide gel electrophoresis (PAGE). (C) Digestion of ATP-33 by T5 Exo in the absence or presence of 250 μM ATP, ADP, AMP, adenosine (Ade), GTP, CTP, or UTP after 16 h. (D) Total concentrations of the parent aptamer and 28-nt major product from the gel in panel C, as calculated relative to corresponding ladder bands. Error bars indicate the standard deviation of two experiments.