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. 2020 Oct 22;48(20):11421–11433. doi: 10.1093/nar/gkaa873

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — MAVS mediated NF-kB activation does not account for the differential effect of IRF3 R285Q. IRF3-deficient HEK293T cells were transfected with either a firefly luciferase reporter fused to 55 base pairs of the IFNB1 promoter followed by several repeats of IRF3 binding sites (A and B) or a firefly luciferase reporter fused to several repeats of NF-kB-binding sites (C and D). The cells were transfected with either IRF3 WT or R285Q and stimulated with MAVS (A and C) or STING and cGAS (B and D). Luciferase activities were measured 24 h post transfection and calculated relative to a constitutively active Renilla luciferase reporter. The data were normalized to the mock sample and presented as triplicates ± SD. Similar data were obtained for three independent experiments. One-way ANOVA test was used for statistical analysis; ns, not significant; *, P ≤ 0.05; ****, P ≤ 0.0001. Protein amounts were analyzed by western blotting (Supplementary Figure S3).