Table 2 –

Hydrogel composition for generation of engineered cardiac tissues using the frame-hydrogel method. V is defined as the volume of cell/hydrogel mixture required for one engineered tissue (in mL) and N_tissues is defined as the number of tissues to make consecutively.

	Starting Concentration	Final Concentration	Percent of Final Hydrogel Volume	Volume to Prepare
Fibrinogen	10 mg/mL	2 mg/mL	20%	0.2×V×Ntissues
Matrigel	100%	10% (v/v)	10%	0.1×V×Ntissues
Thrombin	50 U/mL	1 U/mL	2%	0.02×V×Ntissues
2X Media	100%	20%	20%	0.2×V×Ntissues
Cells suspended in culture media	$\frac{\mathit{\text{Cells\hspace{0.17em}}}per\mathit{\text{\hspace{0.17em}tissue}}}{0.48\times V}\mathit{\text{cells}}/mL$	$\frac{\mathit{\text{Cells\hspace{0.17em}}}per\mathit{\text{\hspace{0.17em}tissue}}}{V}\mathit{\text{cells}}/mL$	48%	0.48×V×Ntissues