Fig. 1.

Effects of adiponectin and resistin on glucose uptake in cultured hippocampal neurons. Neuronal cultures treated with varying doses of adiponectin (a) or resistin (b) for the indicated length of time were exposed to radiolabeled ³H-glucose for 15 s to measure glucose uptake. We also measured the uptake of glucose in initial velocity conditions over a 2-min time frame with 20 ng/mL of adiponectin (c) or 200 pg/mL of resistin (d). The proteins lost their activity upon heat-induced denaturation. The effects of adiponectin (e) and resistin (f) on glucose uptake in the presence of activators/inhibitors of AMPK and AKT. Data represent the mean ± SEM of n = 5, each performed in triplicate. *p < 0.01; **p < 0.005, Bonferroni test