Fig. 3.

Downstream effect of adiponectin and resistin treatment in hippocampal neurons. Afterward, we studied the effect of adiponectin and resistin on PPP activity and did not observe changes after treatment (a). Then, we measured the levels of activity of AMPK, a master metabolic regulator, using a specific calorimetric kit (b) and Western blot (c). Then, we measured the levels of ATP and the ATP/ADP ratio after treatment for 15 min with adiponectin (d and e) and resistin (f and g). Data represent the mean ± SEM of n = 4, each performed in triplicate. *p < 0.01; **p < 0.005, Bonferroni test