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. 2020 Oct 28;21(21):8017. doi: 10.3390/ijms21218017

Figure 2.

Figure 2

Combined effect of AuNP@PEG and irradiation on cell viability measured by CellTox green. (A) HCT116 spheroids incubated for 24 h with 8 nM AuNP@PEG and then irradiated with a 532-nm green laser for 1 min; (B) HCT116 spheroids irradiated with a 532-nm green laser for 1 min; (C) Doxorubicin-resistant HCT116 (HCT116-DoxR) spheroids incubated for 24 h with 8 nM AuNP@PEG and then irradiated with a 532-nm green laser for 1 min; (D) HCT116-DoxR spheroids irradiated with a 532-nm green laser for 1 min. Microscopy images were acquired in Brightfield or with a green fluorescence filter to evaluate CellTox green dye fluorescence, after 30 min, 1 h 30 min or 24 h incubation. The combined images result from the overlap between Brightfield and green filter images. Scale bar corresponds to 100 μm.