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. 2020 Oct 28;21(21):8017. doi: 10.3390/ijms21218017

Figure 6.

Figure 6

Effect of AuNPs and irradiation in Doxorubicin (Dox) diffusion in Dox-resistant HCT116 (HCT116-DoxR) spheroids. (A) HCT116-DoxR spheroids irradiated with a 532-nm laser for 1 min and then incubated with 8 μM Dox; (B) HCT116-DoxR spheroids incubated for 6 h with 8 μM Dox and then irradiated with a 532-nm laser for 1 min; (C) HCT116-DoxR spheroids incubated with 8 nM AuNP@PEG, irradiated with a 532-nm laser for 1 min and then incubated with 8 μM Dox; (D) HCT116-DoxR spheroids incubated with 8 nM AuNPs, incubated for 6 h with 8 μM Dox and then irradiated with a 532-nm laser for 1 min. Microscopy images were acquired in Brightfield or with a red fluorescence filter after 30 min, 1h 30 min or 24h incubation. The combined images result from the overlap between Brightfield and red filter images. Scale bar corresponds to 100 μm.