Figure 4.

DNA damage bypass by Polη during RNA synthesis in the presence of manganese. (A,B) The template in S12 contained 8-oxoG in the incoming position. Reactions were performed for 3 min with 5-mM Mn²⁺, 1.6-nM Polη, 8-nM RNA/DNA primer/template and the indicated amount of individual rNTPs. (C,D) The template in S16 contained TT dimer in the incoming position. Reactions were performed for 15 min with 5-mM Mn²⁺, 1.6-nM Polη, 20-nM RNA/DNA primer/template and the indicated amount of individual rNTPs. RNA primers and rNTPs are highlighted in red.