Figure 5.

Metal ion preference of Polη during RNA synthesis. Primer extension reactions were performed with 10-nM Polη, 20-nM S6 RNA/DNA primer/template and 1-mM rNTP mix for 5 min. (A) Reactions contained both Mn²⁺ and Mg²⁺ in the indicated concentrations. (B) Reactions contained 5-mM Mg and the indicated concentrations of Mn²⁺. Manganese is highlighted in blue. The positions of the primer and its one nucleotide extension (+1) are indicated.