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qPCR validation of hypoxia induced gene expression changes in human astrocytes. qPCR confirmed a significant downregulation of (A) NDUFAF4 (p = 0.001), (B) MFN1 (p = 0.007), (C) SUCLG2 (p = 0.02), (D) DLAT (p = 0.007); and a non-significant upregulation of (E) HLA-DPB1 (p = 0.11) and (F) MT1X (p = 0.08) in response to hypoxia. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
