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Transactivation assay of the CaHsfA1d protein in yeast cells. (A) Schematic representation of the full-length CaHsfA1d and deletion protein constructs in the pGBKT7 vector. (B) Growth of transformed yeast cells 3 days after spotting on selective mediums. Positive control, yeast cells transformed with pGBKT7-53 and pGADT7-T; Negative control, yeast cells transformed with pGBKT7-Lam and pGADT7-T. Experiments were performed three times with similar results.
