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. 2020 Nov 8;21(21):8374. doi: 10.3390/ijms21218374

Figure 5.

Figure 5

Thermotolerance analyses of CaHsfA1d-silenced pepper seedlings. (A) Appearance of TRV2:00 and TRV2:CaHsfA1d pepper plants before heat treatment, after heat treatment, and after recovery. Scale bar = 10 cm. (B) Heat injury index scored daily for TRV2:00 and TRV2:CaHsfA1d pepper plants after heat treatment in Figure 5A. (C) Survival rate of TRV2:00 and TRV2:CaHsfA1d seedlings after recovery in Figure 5A. (D) Trypan blue coloration in the leaves of TRV2:CaHsfA1d pepper plants compared with TRV2:00 after 45 °C treatment for 2 h. Scale bar = 1 cm. (E) The phenotype of leaf discs isolated from TRV2:00 or TRV2:CaHsfA1d plants at 12 h post 37 °C heat stress. Scale bar = 1 cm. (F) Chlorophyll and (G) MDA content of the leaf discs (Figure 5E) exposed to heat stress. Data are means ± SD of three biological replicates. The asterisks on the top of bars indicate significant differences between the TRV2:00 and TRV2:CaHsfA1d plants. * p < 0.05, ** p < 0.01 by t-test.