Figure 6.

Thermotolerance analyses of CaHsfA1d-overexpression Arabidopsis seeds and seedlings. (A) The germination phenotypes and (B) germination rates of transgenic and WT seeds grown at 22 °C for 4 days after preheating at 37 °C for 7 or 10 days. Schematics at the right of Figure 6A show the heat stress regimes of Arabidopsis seeds. (C–E) Performance of the CaHsfA1d-overexpression and WT seedlings under heat stress. Schematics at the top of Figure 6C–E show the heat stress regimes of Arabidopsis seedlings. Data are means ±SD of three biological replicates. The asterisks on the top of bars indicate significant differences between CaHsfA1d transgenic and WT plants. * p < 0.05, ** p < 0.01 by t-test. Scale bar = 1 cm.