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Detection of H2O2 in the leaves of WT and overexpression Arabidopsis lines. (A) H2O2 staining from the leaves of WT and transgenic plants post 37 °C heat treatment for 2 h. (B) Quantification of relative DAB staining of the WT and CaHsfA1d-overexpression Arabidopsis. Data are means ± SD of three biological replicates. The asterisks on the top of bars indicate significant differences between WT and overexpressing lines. * p < 0.05 by t-test. Scale bar = 1 cm.
