Skip to main content
. 2020 Nov 6;21(21):8336. doi: 10.3390/ijms21218336

Figure 4.

Figure 4

Figure 4

Cordycepin suppressed FGF9-induced expression of cyclin D1, cyclin E1, cyclin A1 and cyclin B1 in MA-10 cells. (A) Western blot analysis of cyclin D1, cyclin E1, cyclin A1 and cyclin B1 expression in MA-10 cells treated without or with FGF9 (50 ng/mL) and different concentrations of cordycepin (0, 25, 50 and 100 µM) for 0.25, 12 and 24 h. (B–E) The relative expression of (B) cycline D1, (C) cyclin E1, (D) cyclin A1 and (E) cyclin B1 was quantified using ImageJ software by normalization with β-actin. Values are shown as the mean ± SEM, n = 4. p values were calculated using two-way ANOVA with Tukey’s multiple comparisons post-tests. * p < 0.05, ** p < 0.01, compared to the control group (0 ng/mL FGF9) at each dose of cordycepin; §§ p < 0.01, §§§ p < 0.001 compared to the group with 0 μM cordycepin and 0 ng/mL FGF9 treatments; # p < 0.05, ### p < 0.001 compared to the group with 0 μM cordycepin and 50 ng/mL FGF9 treatments.