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. 2020 Nov 5;21(21):8286. doi: 10.3390/ijms21218286

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FUT6 overexpression in SW620 cells led to increased cell migration and wound healing. (A) Pictures of one of the scratch wound healing assays, performed using culture inserts, from day 0 (left), day 4 (middle) and day 6 (right) with SW620Mock cells (upper) and SW620FUT6 cells (lower) obtained with an inverted microscope. (B) Means with standard deviation of percentage of free areas for 8 independent experiments are represented, free areas were determined from pictures using ImageJ and converted into percentage values. At day 2, 3, 4 and 5, statistical analysis using an unpaired Student’s t-test demonstrated significant higher migration ability for SW620FUT6 cells compared to SW620Mock cells. Differences were considered statistically significant when p < 0.05 (*), and p < 0.01 (**).