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. 2020 Nov 5;21(21):8286. doi: 10.3390/ijms21218286

Figure 4.

Figure 4

SW620FUT6 cells express E-selectin ligands. (A) Membrane proteins (Mb Prot) from SW620Mock and SW620FUT6 cells were stained with E-Ig plus anti-mouse CD62E plus anti-rat IgG (H+L) HRP in PBS with Ca²+ by WB. β-tubulin protein expression level was analyzed as loading control. (B) Membrane proteins, cleared membrane proteins from immunoprecipitated (IP) E-selectin ligands and IP E-selectin ligands (IP E-sel) from 100 µg. of SW620FUT6 cell membranes were stained with rat anti-human HECA-452 mAb followed by goat anti-rat IgM HRP by WB.