Table 2.

List of E-selectin ligands in SW620FUT6 cells identified by mass spectrometry†. † From SW620Mock and SW620FUT6 cells extracted membrane proteins, four immunoprecipitations with E-Ig were performed and analyzed by LC–MS/MS. The present list shows the glycosylated‡ immunoprecipitated E-selectin ligands only identified in SW620FUT6 cells (Supplementary Materials Table S3 for glycosylated‡ IP E-selectin ligands identified in both cell lines). § Proteins identified are described with the number of unique peptides for each experiment and the sum of the total spectrum count from the four experiments. ‡ Protein information on glycosylation status and subcellular location were extracted from the UniProtKB database.

Protein Name	Gene Name	MW (kDa)	UniProtKB Entry	Exclusive Unique Peptide Counts §				Total Spectrum Count (sum) §	Subcellular Location ‡
				Exp. 1	Exp. 2	Exp. 3	Exp. 4
Neural cell adhesion molecule L1	L1CAM	140	P32004	31	20	30	27	118	Plasma membrane
Integrin α6	ITGA6	127	P23229	11	31	12	10	91	Plasma membrane
Receptor-type tyrosine-protein phosphatase eta	PTPRJ	146	Q12913	21	9	18	11	58	Plasma membrane
Integrin β1	ITGB1	88	P05556	6	11	7	5	39	Plasma membrane, recycling endosome
Cation-independent mannose-6-phosphate receptor (Insulin-like growth factor 2 receptor)	IGF2R	274	P11717	16	3	16	7	39	Lysosome membrane
Receptor-type tyrosine-protein phosphatase alpha	PTPRA	91	P18433	10	3	4	5	22	Membrane
Leucyl-cystinyl aminopeptidase	LNPEP	117	Q9UIQ6	7	9	6	5	22	Plasma membrane, secreted
Carboxypeptidase D	CPD	153	O75976	2	0	6	6	17	Plasma membrane
Lysosome-associated membrane glycoprotein 2	LAMP2	45	P13473	3	3	3	3	12	Lysosome/endosome/plasma membrane
CD109 antigen	CD109	162	Q6YHK3	3	2	2	4	12	Plasma membrane
Golgi membrane protein 1	GOLM1	45	Q8NBJ4	2	2	2	4	10	Golgi apparatus membrane
Plexin-D1	PLXND1	212	Q9Y4D7	2	0	1	4	7	Plasma membrane
Zymogen granule protein 16 homolog B	ZG16B	23	Q96DA0	0	2	0	2	4	Secreted

Abbreviation: MW, Molecular Weight; Exp., Experiment.