Phylograms with branch lengths based on amino acid sequences. The tree topologies were fixed to those obtained using third positions of codons for genes encoding five core C enzymes: (a) NADP-malate dehydrogenase (NADP-MDH, gene nadpmdh-1P1), (b) NADP-malic enzyme (NADP-ME, gene nadpme-1P4), (c) phosphoenolpyruvate carboxykinase (PCK, gene pck-1P1), (d) phosphoenolpyruvate carboxylase (PEPC, gene ppc-1P3), and (e) pyruvate phosphate dikinase (PPDK, gene ppdk-1P2). The major taxonomic groups are indicated with shades, and branches from CAndropogoneae are in red. Yellow circles indicate gene duplications. Scale bars = 0.01 expected amino acid substitutions per site (panels a–e are depicted at different scales). C species outside Andropogoneae were pruned from the tree. See Supplementary Fig. S7 available on Dryad for details, and Supplementary Fig. S6 available on Dryad for branch lengths based on nucleotides.