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. 2020 Nov 11;19:1533033820970668. doi: 10.1177/1533033820970668

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Down-regulation of C3aR/C5aR suppressed HCC cell proliferation. MHCC-97H and SK-HEP-1 cells were transfected with si-C3aR and si-C5aR, respectively. A, The protein expression of C3aR/C5aR was detected in MHCC-97H and SK-HEP-1 cells using western blotting. B, CCK-8 assay was conducted to determine cell proliferation in MHCC-97H and SK-HEP-1 cells. C-D, The colony formation was assessed in si-C3aR-transfected MHCC-97H cells and si-C5aR-transfected SK-HEP-1 cells. Data were expressed as mean ± SD. ***p < 0.001, compared with si-NC.