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Assessment of breast cancer cell viability following a single freeze/passive thaw event. The MCF-7 cells were subjected to a 5-minute freeze at −10°C, −15°C, −20°C, or −25°C followed by a 5-minute passive thaw, and survival was assessed. Data suggest that complete cell death with no recovery is attained following exposure to −25°C whereas −15°C and −20°C exposure results in a substantial level of cell death.
