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. 2020 Nov 11;19:1533033820971669. doi: 10.1177/1533033820971669

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Downregulation of DARS-AS1 induces apoptosis of cervical cancer cells through miR-188-5p/HMGB1 axis. (A, B) The protein level of HMGB1 in SiHa cells infected with HMGB1-OE plasmids was analyzed by western blot assay. **P < 0.01 compared with Ctrl-vector group. (C) SiHa cells were transfected with DARS-AS1 siRNA3 with or without HMGB1-OE plasmids for 48 h. Cell viability was analyzed by CCK-8 assay. (D) Apoptotic cells were measured by flow cytometry. (E) Western analysis of HMGB1, p-p38, p-JNK and p-p65 proteins in SiHa cells. (F, G, H, I) The relative expressions of HMGB1, p-p38, p-JNK, p-p65 in SiHa were normalized to β-actin, p38, JNK, p65, respectively. **P < 0.01, compared with the siRNA-ctrl group; ^##P < 0.01 compared with DARS-AS1 siRNA3 group.