Fig. 4.

Staining of actin filaments on HEK293 cells on soft (5 kPa) and rigid (2 MPa) surfaces after treatment with different concentrations (0–1 μg/μL) of MNPs@SiO₂(RITC) for 12 h. For actin labeling, cells were incubated with Alexa Fluor 488-conjugated phalloidin (Molecular Probe, Eugene, OR, USA), (1:200) diluted in PBS, for 1 h at room temperature