Table 2.
Effects of Tat and morphine on microglial morphology in the striatum and spinal cord of Tat transgenic mice
| CNS region | Measure | Genotype | Repeated saline | Repeated morphine | Genotype effect | Drug effect | Genotype x drug | |||
|---|---|---|---|---|---|---|---|---|---|---|
| mean ± SEM | mean ± SEM | F1, 56 | p | F1, 56 | p | F1, 56 | p | |||
| Striatum | Soma area (μm2) | Tat(−) | 51.1 ± 2.16 | 46.7 ± 2.98 | < 1.0 | 0.49 | 2.9 | 0.09 | < 1.0 | 0.96 |
| Tat(+) | 49.2 ± 2.41 | 45.1 ± 2.00 | ||||||||
| Maximum branch length (μm) | Tat(−) | 35.3 ± 3.32 | 32.5 ± 2.59 | < 1.0 | 0.59 | < 1.0 | 0.84 | 1.7 | 0.20 | |
| Tat(+) | 30.7 ± 1.90 | 34.4 ± 2.19 | ||||||||
| Critical radius (μm) | Tat(−) | 14.6 ± 1.56 | 13.4 ± 1.35 | < 1.0 | 0.51 | < 1.0 | 0.97 | < 1.0 | 0.63 | |
| Tat(+) | 14.4 ± 1.76 | 15.6 ± 1.20 | ||||||||
| Number of primary process | Tat(−) | 4.8 ± 0.56 | 4.1 ± 0.30 | 2.8 | 0.10 | < 1.0 | 0.92 | 2.8 | 0.10 | |
| Tat(+) | 3.5 ± 0.29 | 4.1 ± 0.45 | ||||||||
| Process maximum | Tat(−) | 6.8 ± 0.83 | 5.3 ± 0.60 | < 1.0 | 0.93 | < 1.0 | 0.70 | 2.3 | 0.13 | |
| Tat(+) | 5.5 ± 1.00 | 6.4 ± 0.77 | ||||||||
| Process total | Tat(−) | 21.5 ± 3.02 | 16.1 ± 2.20 | < 1.0 | 0.89 | < 1.0 | 0.70 | 2.1 | 0.16 | |
| Tat(+) | 16.9 ± 3.49 | 19.9 ± 2.77 | ||||||||
| Spinal cord | Soma area (μm2) | Tat(−) | 48.5 ± 3.42 | 50.9 ± 3.79 | 4.2 | 0.04 | 3.8 | 0.06 | 1.5 | 0.23 |
| Tat(+) | 51.4 ± 2.45 | 62.2 ± 3.73 | ||||||||
| Maximum branch length (μm) | Tat(−) | 35.1 ± 2.59 | 36.2 ± 2.71 | < 1.0 | 0.51 | < 1.0 | 0.66 | < 1.0 | 0.98 | |
| Tat(+) | 33.4 ± 2.32 | 34.6 ± 2.19 | ||||||||
| Critical radius (μm) | Tat(−) | 16.7 ± 1.98 | 15.6 ± 1.20 | < 1.0 | 0.44 | < 1.0 | 0.98 | < 1.0 | 0.61 | |
| Tat(+) | 17.2 ± 2.28 | 18.1 ± 2.04 | ||||||||
| Number of primary process | Tat(−) | 2.3 ± 0.28 | 2.7 ± 0.33 | < 1.0 | 0.81 | 1.5 | 0.23 | 5.0 | 0.03 | |
| Tat(+) | 3.2 ± 0.47 | 2.0 ± 0.20 | ||||||||
| Process maximum | Tat(−) | 3.6 ± 0.31 | 4.6 ± 0.57 | < 1.0 | 0.81 | < 1.0 | 0.78 | 5.4 | 0.02 | |
| Tat(+) | 4.8 ± 0.58 | 3.6 ± 0.27 | ||||||||
| Process total | Tat(−) | 11.5 ± 1.56 | 15.7 ± 2.30 | < 1.0 | 0.58 | < 1.0 | 0.95 | 5.1 | 0.03 | |
| Tat(+) | 14.6 ± 1.75 | 10.6 ± 1.22 | ||||||||
Sholl analysis of microglial morphology in the striatum and spinal cord of repeated (8-day) saline- or morphine-treated Tat(−) and Tat(+) mice. Data are expressed as the mean ± SEM. The parameters measured by Sholl analysis are indicated in parentheses in the second column. One-way ANOVAs for each CNS region were conducted with genotype and drug as between-subjects factors. F values and p values are presented from ANOVA results. Bolded values denote significant differences at α = 0.05; mean ± SEM, n = 3–4 mice per group/4 sections each