HP, CS, and DS hexasaccharide-induced changes in the 15N-edited HSQC spectrum of PTN. HSQC spectra of PTN in the presence of different concentrations of GAG hexasaccharides were acquired and superimposed. The concentration of the PTN was 0.1 mM. The concentrations of GAG hexasaccharides were 0 (dark green), 0.1 (brown), 0.2 (blue), 0.4 (pink), 0.6 (light green), and 0.8 (yellow) mM, respectively. All GAGs induced similar changes in the chemical shifts of PTN’s backbone amide nitrogens and hydrogens.