Table 1.
Primary and secondary antibodies and RNAscope probe used
| Antibodies and RNAscope probe | Vendor | Catalog # |
|---|---|---|
| Primary antibodies | ||
| Rabbit anti Piezo2 | Novus Biologicals, Centennial, CO | NBPI-78624 |
| Mouse anti-neuronal class III β-tubulin | BioLegend, San Diego, CA | 801201 |
| Chicken anti-GFP | Abcam, Cambridge, MA | ab13970 |
| Goat anti-TRPV1 | Neuromics, Edina, MN | GT15129 |
| Secondary antibodies | ||
| Donkey anti-rabbit IgG Alexa Fluor 555 | Abcam, Cambridge, MA | ab150078 |
| Donkey anti-rabbit IgG Alexa Fluor 647 | Invitrogen, Thermo Fisher Scientific, Eugene, OR | A31573 |
| Goat anti-mouse IgG Alexa Fluor 488 | Invitrogen, Thermo Fisher Scientific, Eugene, OR | A11029 |
| Goat anti-chicken IgG Alexa Fluor 647 | Invitrogen, Thermo Fisher Scientific, Eugene, OR | A21449 |
| Goat anti-chicken IgG Alexa Fluor 488 | Invitrogen, Thermo Fisher Scientific, Eugene, OR | A11039 |
| Goat anti-chicken IgG Alexa Fluor 555 | Invitrogen, Thermo Fisher Scientific, Eugene, OR | A21437 |
| Goat anti-rabbit IgG Alexa Fluor 647 | Invitrogen, Thermo Fisher Scientific, Eugene, OR | A32733 |
| Donkey anti-goat IgG Alexa Fluor 647 | Abcam, Cambridge, MA | ab105131 |
| Goat anti-mouse IgG Alexa Fluor 555 | Invitrogen, Thermo Fisher Scientific, Eugene, OR | A32727 |
| Goat anti-rabbit IgG Alexa Fluor 488 | Invitrogen, Thermo Fisher Scientific, Eugene, OR | A11008 |
| RNAscope probe Mm-Piezo2 | ACDBio | 400191 |