Table 2.
Membrane potential (mV) | Rheobase (pA) | Input resistance (MΩ) | Mechanical threshold (µm) | Current amplitude (pA/pF) | Cell capacitance (pF) | ||
---|---|---|---|---|---|---|---|
WT | RA | −61.5 ± 1.4 (n = 75) | 407 ± 36 (n = 45) | 89.1 ± 9.8 (n = 71) | 6 ± 0.3 (n = 75) | 7.4 ± 1.4 (n = 75) | 40 ± 2.6 (n = 75) |
IA | −65.7 ± 1.5 (n = 18) | 486 ± 86 (n = 10) | 88.5 ± 18.6 (n = 18) | 6.6 ± 0.8 (n = 18) | 6.7 ± 1.9 (n = 18) | 43.7 ± 6.4 (n = 18) | |
SA | −62.9 ± 1.7 (n = 22) | 401 ± 59 (n = 15) | 104 ± 23.5 (n = 22) | 5.6 ± 0.6 (n = 22) | 15.9 ± 3.8* (n = 22) | 39.2 ± 5.8 (n = 22) | |
Piezo2cKO | RA | −58.4 ± 2.9 (n = 14) | 401 ± 85 (n = 8) | 56.1 ± 5.9 (n = 14) | 3.9 ± 0.9# (n = 14) | 5.8 ± 1.6 (n = 14) | 39.9 ± 6 (n = 14) |
IA | −81.1 (n = 1) | 322 (n = 1) | 40 (n = 1) | 11 (n = 1) | 2.3 (n = 1) | 21.3 (n = 1) | |
SA | −60.8 ± 5.4 (n = 4) | 355 (n = 1) | 55.3 ± 12.6 (n = 4) | 5.5 ± 1.5 (n = 4) | 6.7 ± 3.8 (n = 4) | 47.4 ± 12.5 (n = 4) |
Electrical properties of mechanosensitive corneal neurons from wild-type and Piezo2cKO mice. Mean ± SE values are given for each parameter. Membrane potential values were adjusted for the calculated liquid junction potential of +12 mV. To compare the means of different parameters among the three types of MA cells, the Kruskal–Wallis one-way ANOVA on ranks test and the Dunn's method one-way ANOVA test were used.
*Density of current in SA cells is significantly higher than in RA cells in wild-type mice (p = 0.037). To compare the means of different parameters for each type of cell between wild-type and Piezo2cKO mice, the Student's t test and Mann–Whitney rank-sum test were used.
#The mechanical threshold in RA cells from Piezo2cKO mice is lower than in RA cells from wild-type mice (p = 0.013). The absence of symbols indicates the lack of significant differences.