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. 2020 Nov 17;11(11):991. doi: 10.1038/s41419-020-03162-w

Fig. 6. Mitochondrial respiration chain deficiency inhibits lysosomal degradation.

Fig. 6

a VSMCs were transfected with UQCRC1 siRNA or NC-siRNA for 48 h. Then, cells were harvested, and the protein levels of UQCRC1 were analysed by western blotting. N = 3 independent experiments. b MMP changes were monitored by flow cytometry using JC-1 staining. N = 5 independent experiments. c Mitochondrial ROS production was evaluated by confocal microscopy using the fluorescent probe MitoSOX. Scale bars = 10 μm. N = 40–50 cells per group. d Lysosomal degradation was assessed using self-quenched BODIPY FL conjugate of BSA. Scale bars = 10 μm. N = 40–50 cells per group. e Representative confocal images show LDHB (green channel), v-ATPase V0A1 (red channel), and merged images (yellow channel) puncta in siRNA-UQCRC1-treated VSMCs. Scale bars = 10 μm. N = 40–50 cells per group. f Protein expression of LDHB and v-ATPase V0A1 was detected by western blotting. N = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.