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. 2020 Nov 17;10:19943. doi: 10.1038/s41598-020-77061-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In situ hybridization for candidate hormone receptor genes in the vomeronasal organ of adult virgin male mice. (a–n) Each panel shows chromogenic in situ hybridization staining (purple) on VNO coronal sections with probes for genes Gpr12 (a), Gpr63 (b), Ackr3 (c), Htr1b (d), P2ry14 (e), Tacr1 (f), Galr2 (g), Gpr19 (h), Npy1r (i), Kiss1r (j), Avpr1a (k), and Oxtr (m). Positive control (l) is staining with probe for V2R vomeronasal receptor gene Vmn2r46, expected to be expressed in a large set of cells in the VNO's basal zone. For the oxytocin receptor gene Oxtr, staining obtained with a control sense probe is shown for comparison (n). ep, vomeronasal sensory epithelium; lu, vomeronasal organ lumen. Bar is 100 μm. (o) Glomerular layer of the accessory olfactory bulb from Oxtr::Gfp transgenic mice where GFP is under the control of the Oxtr gene promoter (GENSAT Project database⁵⁶). Immunostaining for GFP is shown in brown. Higher magnification image on the right shows anterior and posterior divisions of the bulb.