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. 2020 Nov 17;10:19943. doi: 10.1038/s41598-020-77061-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confirmation of expression of oxytocin receptor gene Oxtr and vasopressin receptor gene Avpr1a in the VNO by quantitative PCR and in situ hybridization. Left panels: relative expression quantitation (RQ) determined by RT-qPCR with TaqMan probes for Oxtr (a) and Avpr1a (b) in the VNO (n = cDNAs from 3 animals (biological replicates) per group, 3 technical replicates per biological replicate). RQ values are relative to mean expression level of the ‘virgin’ male group. Right panels: representative images of in situ hybridization experiments for Oxtr (a) and Avpr1a (b) on VNO coronal sections from virgin and dpc22 animals. ep vomeronasal sensory epithelium, lu vomeronasal organ lumen. Bar is 100 μm.